Three dimensional rapid diffusion tensor microimaging for anatomical characterization and gene expression mapping in the mouse brain
نویسندگان
چکیده
mouse embryo. In original 2D ISH sections (a), it is difficult to comprehend the distributions of Lhx8 (blue) and Shh (brown) genes relative to each other and to brain structures. After mapping, coregistered Lhx8 (orange) and Shh (green) expression data are overlaid on the iDW image (b), and inspected in 3D with brain structures e.g. ventricles (blue) to appreciate the spatial expression patterns of the two genes (c). Fig. 1: DTMI of an adult mouse brain at 55 μm resolution. A) iDW and DEC contrasts in a horizontal brain section. B-C) Comparison of anatomical details revealed by DTI at 55 μm (B,C) and 125 μm (B',C') resolutions. DTMI at 55 μm revealed the microstructural organization of white matter fibers in the striatum (white arrows), that were not seen at lower resolutions (right). Scale bars=1mm. Three dimensional rapid diffusion tensor microimaging for anatomical characterization and gene expression mapping in the mouse brain
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